Sridevi, 49, will shoot for KBC in Mumbai Tuesday, a source told IANS.
She will be on the show as part of a promotional stint for "English Vinglish", in which Big B has played a cameo.
Big B and Sridevi have earlier shared the frame in movies like "Aakhree Raasta", "Inquilab" and "Khuda Gawah".
After shooting his cameo for "English Vinglish", Amitabh had tweeted Sridevi is "still the same... effervescent, spontaneous, delightful".
Directed by debutante director Gauri Shinde, wife of "Paa" director R.Balki, "English Vinglish" is set to release Oct 5.