"I request the centre to ban the film. The film should not be allowed to release and if screened, human rights organisations should protest against it in cities like Mumbai," Vaiko said in a statement.
"I also urge the Tamil Nadu government to ban the film in the state," he added.
When the film's trailer was release in July, the Tamil activist group Naam Tamizhar lead by filmmaker Seeman sought a ban on the film on the grounds of portraying LTTE cadres as terrorist.
On Sunday, a special screening of the dubbed version of "Madras Cafe" was organized for the group, but they are sticking to their guns.
Seeman said: "The film tries to portray late LTTE chief Prabhakaran as a terrorist. We don't appreciate it and request the distributors not to screen the film in the state. The sentiment of the film is anti-Tamil and we don't agree with it".