The young actor, who was in hometown Chandigarh, donated blood at a camp in his alma mater Panjab University.
Ayushmann was the first to donate blood at the camp organised by the university's English department.
The actor, who had donated blood five times before, also inspired students to join the voluntary blood donation movement.
The actor shot into fame some months ago with the success of "Vicky Donor", which dealt with the taboo subject of sperm donation.
Ayushmann completed his post-graduation from Panjab University's School of Communication Studies a few years ago before becoming a radio jockey and a TV show host.
Nearly 70 students, mostly women, also donated blood at the camp, the department's chairperson Harpreet Pruthi said.